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qPCR Assay Development and eDNA Testing
An environmental consulting company approached Pisces Molecular in early 2020 with a significant challenge for a lab of our small…
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Environmental DNA sample collection
While the basic requirements of environmental DNA (eDNA) sample collection are simple – pass water (or air) through a micron…
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Plasmid-based qPCR positive controls
Over time, qPCR assays have steadily superseded PCR assays, both for their ability to provide quantitation of target copy number,…
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“Instant” temporal studies from archived
Right from Pisces’ beginning we have archived all sample DNA extracts. Our typical spin-column DNA extraction procedure gives a final…
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Testing multi-worm samples of Tubifex
As a part of their work on Trout Whirling Disease, Beauchamp, et al. determined that there were four sub-species or…
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Skin swab pooling for chytrid testing
In the early days of chytrid fungus testing (ca. 2007), there was a need for relatively large-scale screening of locations…
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Improving a Freshwater Seal qPCR Assay
In this project we modified an existing qPCR assay for Freshwater Seals, Phoca vitulina mellonae used for phylogenetic studies of…
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Greenback Cutthroat Trout in Colorado
Pisces played an important role in the discovery and reestablishment of Greenback Cutthroat trout in Colorado. The story of the…
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Determining Paternity and Sex in Komodo
In 2019, herpetologists from the Chattanooga Zoo in Tennessee contacted Pisces with an interesting question. Their female Komodo Dragon (Varanus…
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